14974   Slc16a4     Status: ES cell colonies screened / QC positives

GeneSlc16a4
Chromosome3
Deletion Start107,292,442
Deletion End107,311,647
Deletion Size19,206
Genome Build38
MGI IDMGI:2385183
NCBI Gene ID229699
BAC ID419B5
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 3

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF107,292,629107,292,648AGCCAGGAACTTGCCCAAAC
TUP107,292,653107,292,676AAGATCAGAGGCCACCTTGGCTGA
TUR107,292,682107,292,702GGGAACAAACAGCTCAGCTTC
TDF107,311,293107,311,317ACAGGTGAGAAGCCCTATTTAAGTC
TDP107,311,321107,311,350TGGCTCACAATATGGACTGAGAACAGGAGA
TDR107,311,420107,311,440CCAGCCTGCAAGGTAGGAATG

PCR Primers

PrimerSequence
SUATTCAAGGGCTGGTTCACAG
SDTGGTGACAGAATTACCATAGCAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR74 bp++-
TDFTDR148 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
14974A-B11VGB6C57BL/6NTac
14974A-F4VGB6C57BL/6NTac
14974A-H5VGB6C57BL/6NTac
14974A-F10VGB6C57BL/6NTac
14974A-G9VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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