14975   Slc16a5     Status: ES cell colonies screened / QC positives

GeneSlc16a5
Chromosome11
Deletion Start115,462,612
Deletion End115,472,949
Deletion Size10,338
Genome Build38
MGI IDMGI:2443515
NCBI Gene ID217316
BAC ID400H22
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF115,463,143115,463,165GCCATCTCCCAGGACTTTCTATC
TUP115,463,168115,463,193ACAGTAAGGAGACAACAGCCAGCACC
TUR115,463,217115,463,236ACCCACGAGGTCTCTGTCAC
TDF115,472,356115,472,375GCAAGGTCAGGGCTGAGAAC
TDP115,472,377115,472,396TGGCTTGGACTCCGCCAGCC
TDR115,472,404115,472,422TTGGAGGCCGGAGAAACAG

PCR Primers

PrimerSequence
SUGAATTCTGCTGCCCTACCTG
SDTTCATCTGTGCCTGCTATGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR94 bp++-
TDFTDR67 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
14975A-H7VGB6C57BL/6NTac
14975A-G9VGB6C57BL/6NTac
14975A-B12VGB6C57BL/6NTac
14975A-A10VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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