| Gene | Slc19a3 |
| Chromosome | 1 |
| Deletion Start | 83,026,375 |
| Deletion End | 83,013,819 |
| Deletion Size | 12,557 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,026,240 | 83,026,260 | TGTCAGAACCCAGCAAGAATC |
| TUP | 83,026,214 | 83,026,238 | CACCAGCCCAGAGGTAAGGTAACGG |
| TUR | 83,026,140 | 83,026,162 | CGTGGGCTCACTAGAATGACATC |
| TDF | 83,014,789 | 83,014,807 | GCCCTGGTGTTCGGCATAG |
| TDP | 83,014,753 | 83,014,780 | ATCGCCTTGGTGATTCAGACCATCATGA |
| TDR | 83,014,716 | 83,014,735 | GGCAGTTGGAGCCCTCTTTG |
PCR Primers
| Primer | Sequence |
| SU | ATTCTGGAACCCATGCAAAG |
| SD | GGCCATATACGGAGTGCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 121 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14976A-B12 | VGB6 | C57BL/6NTac | |
| 14976A-E11 | VGB6 | C57BL/6NTac | |
| 14976A-F11 | VGB6 | C57BL/6NTac | |
| 14976A-A2 | VGB6 | C57BL/6NTac | |
