14978   Slc20a1     Status: ES cell colonies screened / QC positives

GeneSlc20a1
Chromosome2
Deletion Start129,199,722
Deletion End129,210,917
Deletion Size11,196
Genome Build38
MGI IDMGI:108392
NCBI Gene ID20515
BAC ID387C16
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF129,199,771129,199,790CCGCTCCACCGAAGTATGAC
TUP129,199,795129,199,821TATGGATGCTCATCCTGGGCTTCATCA
TUR129,199,830129,199,849CCCACGGAGAATGCCAAGAC
TDF129,210,802129,210,824CCGCTATCATGGCAGTATTCAAG
TDP129,210,826129,210,848ACATCATCCTGCCAGTGTGACGC
TDR129,210,856129,210,877CCCAGACACTGACACAGCTTTC

PCR Primers

PrimerSequence
SUGGATGAACTTGCGTCCTTTC
SDTACATGCCATCAAATGGCTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR79 bp++-
TDFTDR76 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
14978A-G10VGB6C57BL/6NTac
14978A-A11VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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