| Gene | Slc20a2 |
| Chromosome | 8 |
| Deletion Start | 22,535,287 |
| Deletion End | 22,568,479 |
| Deletion Size | 33,193 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,535,489 | 22,535,511 | CCATTCGGAAAGGCATCATTGAC |
| TUP | 22,535,517 | 22,535,542 | TCTCTACAACGAGACCGTGGAAACGC |
| TUR | 22,535,573 | 22,535,593 | CAATGGAGGGAGGCTACTCAC |
| TDF | 22,567,904 | 22,567,926 | AAGGAGGATCGTATCTGGCAAAG |
| TDP | 22,567,929 | 22,567,956 | TCAAGCATCTGCTGACAGGTAGAAAGGA |
| TDR | 22,567,976 | 22,567,995 | TCTCCAGTCGTGCAGCATAG |
PCR Primers
| Primer | Sequence |
| SU | TACCACTGACTGACCGTAAC |
| SD | GGAGCGCATTCTTCAGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14979A-D12 | VGB6 | C57BL/6NTac | |
| 14979A-D11 | VGB6 | C57BL/6NTac | |
