| Gene | Slc22a18 |
| Chromosome | 7 |
| Deletion Start | 143,476,201 |
| Deletion End | 143,499,298 |
| Deletion Size | 23,098 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 143,476,260 | 143,476,279 | AGCCCTGGAGCTAACGTGTC |
| TUP | 143,476,283 | 143,476,309 | TCATGCAGTTCTCCATCCTACCAGTGA |
| TUR | 143,476,339 | 143,476,357 | CCAGCAGGAAGGCAGTGTG |
| TDF | 143,498,660 | 143,498,681 | TCAGTACCGTTTGGAGAAAGTG |
| TDP | 143,498,682 | 143,498,704 | TGAGAGCCAGCACACTGGATGGA |
| TDR | 143,498,731 | 143,498,751 | TCCAGACCTGTTCCTGTAGAC |
PCR Primers
| Primer | Sequence |
| SU | ACATCATCCCTGTAGCCACC |
| SD | CCAGTGTCCCTGGTGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14983A-A3 | VGB6 | C57BL/6NTac | |
| 14983A-A7 | VGB6 | C57BL/6NTac | |
| 14983A-B10 | VGB6 | C57BL/6NTac | |
| 14983A-D10 | VGB6 | C57BL/6NTac | |
