| Gene | Slc25a29 |
| Chromosome | 12 |
| Deletion Start | 108,835,682 |
| Deletion End | 108,826,644 |
| Deletion Size | 9,039 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,834,878 | 108,834,901 | GACTGTCACCAAGTCTTTCTTCTG |
| TUP | 108,834,846 | 108,834,870 | TGGGAAGGCCACAGAGATTTCCACA |
| TUR | 108,834,813 | 108,834,837 | GGCTCTTTCTGGGTCTCTAATTCTC |
| TDF | 108,826,864 | 108,826,882 | CGTGCCTTCCCGGTCAATG |
| TDP | 108,826,839 | 108,826,863 | CTGCCACCTTTGCCACAGTCACTGT |
| TDR | 108,826,787 | 108,826,807 | AGCTGCCTCACTGTCTACCTG |
PCR Primers
| Primer | Sequence |
| SU | TCGCTTACCGATTGGTTGCG |
| SD | TGACTCTGCAACACGGAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14993A-H5 | VGB6 | C57BL/6NTac | |
| 14993A-F4 | VGB6 | C57BL/6NTac | |
| 14993A-B10 | VGB6 | C57BL/6NTac | |
| 14993A-B4 | VGB6 | C57BL/6NTac | |
| 14993A-G12 | VGB6 | C57BL/6NTac | |
