| Gene | Slc25a3 |
| Chromosome | 10 |
| Deletion Start | 91,123,699 |
| Deletion End | 91,116,948 |
| Deletion Size | 6,752 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 91,123,067 | 91,123,089 | GGGTTGGGAATCTCTTCCTTTTG |
| TUP | 91,123,039 | 91,123,066 | TGCTACTGGTTGGTGAAGGAGGTGACTT |
| TUR | 91,123,014 | 91,123,038 | GGAATGCACACCTCATATAATCTGC |
| TDF | 91,117,261 | 91,117,285 | TCTGTGGTCTCTGTGCTGAATAAAG |
| TDP | 91,117,239 | 91,117,260 | AGAAAGGCAGCACCGCGTCTCA |
| TDR | 91,117,199 | 91,117,220 | GGAGCCTGACTCACCTCTGAAG |
PCR Primers
| Primer | Sequence |
| SU | GGGATAGGGTTGCTAGGAGG |
| SD | GGGACAATTTAGGAATAGCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14994A-C4 | VGB6 | C57BL/6NTac | |
| 14994A-C10 | VGB6 | C57BL/6NTac | |
| 14994A-F2 | VGB6 | C57BL/6NTac | |
| 14994A-F10 | VGB6 | C57BL/6NTac | |
| 14994A-G7 | VGB6 | C57BL/6NTac | |
