| Gene | Slc25a43 |
| Chromosome | X |
| Deletion Start | 36,743,747 |
| Deletion End | 36,777,267 |
| Deletion Size | 33,521 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,744,041 | 36,744,060 | CGGGAGATCGGACTGGAATG |
| TUP | 36,744,062 | 36,744,090 | CAAAGCTGCCACCCTGATGAATCCTAAGA |
| TUR | 36,744,092 | 36,744,113 | ACAGAGGAAGGGAAACTGAGAC |
| TDF | 36,776,557 | 36,776,581 | TCCCGGCTATCATCTTAATTTACTC |
| TDP | 36,776,582 | 36,776,610 | TGGTGTAACTCCATTGCAGAAGTTCCTTC |
| TDR | 36,776,631 | 36,776,652 | GAAGCTGCCCTCAACTGAACTC |
PCR Primers
| Primer | Sequence |
| SU | GAAGATAGACAGGCGAAGGG |
| SD | CTGTGCATGGTGCTTCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14997A-A7 | VGB6 | C57BL/6NTac | |
| 14997A-B9 | VGB6 | C57BL/6NTac | |
| 14997A-C10 | VGB6 | C57BL/6NTac | |
| 14997A-E9 | VGB6 | C57BL/6NTac | |
| 14997A-E10 | VGB6 | C57BL/6NTac | |
| 14997A-G6 | VGB6 | C57BL/6NTac | |
