| Gene | Slc25a45 |
| Chromosome | 19 |
| Deletion Start | 5,878,658 |
| Deletion End | 5,885,005 |
| Deletion Size | 6,348 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,879,009 | 5,879,031 | CCTTTGGAAATCTGCCCTACCTG |
| TUP | 5,879,032 | 5,879,053 | TCAATGGGTGCACTCCTGAGCC |
| TUR | 5,879,056 | 5,879,077 | TGCGTTCACAGTGTCCTTTCTC |
| TDF | 5,884,066 | 5,884,086 | CATGTGGTCCTCCCTGCAATC |
| TDP | 5,884,087 | 5,884,110 | AGGAAGCCCAGGAGCCTAGTCCCA |
| TDR | 5,884,147 | 5,884,165 | GGCTCAGCACTGGACTCTC |
PCR Primers
| Primer | Sequence |
| SU | TGGGAATGAATGGGAATTTG |
| SD | ACTCAAGAGCTGGGTGATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14999A-A4 | VGB6 | C57BL/6NTac | |
| 14999A-E12 | VGB6 | C57BL/6NTac | |
| 14999A-E11 | VGB6 | C57BL/6NTac | |
| 14999A-D9 | VGB6 | C57BL/6NTac | |
| 14999A-D8 | VGB6 | C57BL/6NTac | |
