| Gene | Slc31a2 |
| Chromosome | 4 |
| Deletion Start | 62,286,572 |
| Deletion End | 62,297,239 |
| Deletion Size | 10,668 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,287,433 | 62,287,452 | TACGGTCCCTCCTCTGTGAG |
| TUP | 62,287,454 | 62,287,474 | TGCCGCCCTGCATAAAGCCTC |
| TUR | 62,287,517 | 62,287,537 | ATGCAAGGGCAGAACCCTATC |
| TDF | 62,296,378 | 62,296,399 | GCAAGTGTACTAAAGCACGAAC |
| TDP | 62,296,400 | 62,296,423 | TGCCATCAGCCTGGTATCCCAGTT |
| TDR | 62,296,443 | 62,296,462 | TGGGACCAGAGCACAGAAAG |
PCR Primers
| Primer | Sequence |
| SU | CTAATTGCCAAGGCAAGGTC |
| SD | ATCCTCATGTGTTCCAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15001A-B6 | VGB6 | C57BL/6NTac | |
| 15001A-H11 | VGB6 | C57BL/6NTac | |
| 15001A-E12 | VGB6 | C57BL/6NTac | |
| 15001A-F6 | VGB6 | C57BL/6NTac | |
| 15001A-H10 | VGB6 | C57BL/6NTac | |
| 15001A-G10 | VGB6 | C57BL/6NTac | |
