| Gene | Slc35b4 |
| Chromosome | 6 |
| Deletion Start | 34,176,851 |
| Deletion End | 34,158,373 |
| Deletion Size | 18,479 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,176,244 | 34,176,263 | CTGGGCATTTCGCACATCAC |
| TUP | 34,176,215 | 34,176,241 | TCTTTCGTTGAGAAGCGTGCTCTTTGA |
| TUR | 34,176,179 | 34,176,199 | CACACCACAAAGCCAATCCTG |
| TDF | 34,158,559 | 34,158,578 | CCTCACCGTCACACTGGTTG |
| TDP | 34,158,534 | 34,158,558 | TGACTCTGCGCAAGTTTGTGAGCCT |
| TDR | 34,158,489 | 34,158,511 | TGCCACATAGTGAACTGGTTCTG |
PCR Primers
| Primer | Sequence |
| SU | AGTGCGACACCGACTCACG |
| SD | AAGGCATCGGTAGCAACAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15004A-A1 | VGB6 | C57BL/6NTac | |
| 15004A-A3 | VGB6 | C57BL/6NTac | |
| 15004A-B5 | VGB6 | C57BL/6NTac | |
| 15004A-B8 | VGB6 | C57BL/6NTac | |
| 15004A-F5 | VGB6 | C57BL/6NTac | |
| 15004A-H7 | VGB6 | C57BL/6NTac | |
