| Gene | Slc35f3 |
| Chromosome | 8 |
| Deletion Start | 126,298,789 |
| Deletion End | 126,321,318 |
| Deletion Size | 22,530 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,299,553 | 126,299,574 | ACGGAGGAGGGTTGTGAGTTAC |
| TUP | 126,299,582 | 126,299,606 | CAGCTGTCATTCTGCTGGCCTGAAA |
| TUR | 126,299,636 | 126,299,658 | CAGGAGGAGCTGAGAGCTAAGAG |
| TDF | 126,321,048 | 126,321,067 | AGCTGGGACAGAGGCTGGTG |
| TDP | 126,321,070 | 126,321,094 | AGGACAAGCCGGCACAACTGGACAT |
| TDR | 126,321,134 | 126,321,153 | GCACACGCAGAACACGACAG |
PCR Primers
| Primer | Sequence |
| SU | TCATGGAAGGGCTCATGGAG |
| SD | TATGCCTCTGAGACCTACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15010A-B1 | VGB6 | C57BL/6NTac | |
| 15010C-B2 | VGB6 | C57BL/6NTac | |
| 15010C-G1 | VGB6 | C57BL/6NTac | |
