15011   Slc35f5     Status: ES cell colonies screened / QC positives

GeneSlc35f5
Chromosome1
Deletion Start125,561,248
Deletion End125,591,109
Deletion Size29,862
Genome Build38
MGI IDMGI:1921400
NCBI Gene ID74150
BAC ID169B8
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 1

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF125,561,797125,561,820GGGTAACCTTGAACTTCTGTCTTC
TUP125,561,823125,561,847CCTTACAGGCATGCACCAGCACACC
TUR125,561,870125,561,890GGTGTCGCTCAGCTAGTAGAG
TDF125,590,434125,590,452GCGTCTGGGTGTAGCTGTG
TDP125,590,454125,590,479TGTTCTGTAACCTCAGCAGCTCAGGA
TDR125,590,516125,590,535GGCTCGCTCTCCCTCTGTAG

PCR Primers

PrimerSequence
SUTGGAGTCTGACTGGCTGAC
SDCCTCAAGCAACACATTCGAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR94 bp++-
TDFTDR102 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
15011A-A6VGB6C57BL/6NTac
15011B-E10VGB6C57BL/6NTac
15011B-H5VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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