| Gene | Slc38a1 |
| Chromosome | 15 |
| Deletion Start | 96,624,074 |
| Deletion End | 96,609,861 |
| Deletion Size | 14,214 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,624,012 | 96,624,032 | ATGACGGTGCCCGAAGATGAC |
| TUP | 96,623,980 | 96,624,009 | CGTCAGCAACGACTCTAATGACTTCACAGA |
| TUR | 96,623,942 | 96,623,964 | GGGAAGACGCTCACCTATTTATC |
| TDF | 96,610,015 | 96,610,038 | GAAAGGCTGTGGCTTGAAGAAATG |
| TDP | 96,609,986 | 96,610,012 | TTGAGACCATGTGCTTAGTGACGGTGA |
| TDR | 96,609,953 | 96,609,974 | GGGAGGTTGTTCCTGGAATCTG |
PCR Primers
| Primer | Sequence |
| SU | TGTGAGCTAAAGGTTATGGGATG |
| SD | TTAGAGAAAGCAAGCACCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15012A-A8 | VGB6 | C57BL/6NTac | |
| 15012A-F6 | VGB6 | C57BL/6NTac | |
