| Gene | Slc38a5 |
| Chromosome | X |
| Deletion Start | 8,271,486 |
| Deletion End | 8,279,889 |
| Deletion Size | 8,404 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,271,804 | 8,271,824 | GCTCCTCTGCCCTTCTAACTC |
| TUP | 8,271,825 | 8,271,852 | TCCTCACTTTCTAGTTCCTTCGCTCCAC |
| TUR | 8,271,881 | 8,271,902 | TTCCTGCATTTCCATCCTGTTG |
| TDF | 8,279,066 | 8,279,084 | TGACTGCGGTCCCTTTCTG |
| TDP | 8,279,085 | 8,279,107 | TCCTTCAGGGTCCACTTCAGCCC |
| TDR | 8,279,113 | 8,279,136 | GACACTGGGAAGGATGAAGATGAG |
PCR Primers
| Primer | Sequence |
| SU | ATTCGCATACCGGTCAGTTC |
| SD | TAACACACGGCTCAGACCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15016A-E10 | VGB6 | C57BL/6NTac | |
| 15016A-F5 | VGB6 | C57BL/6NTac | |
| 15016A-F11 | VGB6 | C57BL/6NTac | |
| 15016A-G10 | VGB6 | C57BL/6NTac | |
