| Gene | Slc38a7 |
| Chromosome | 8 |
| Deletion Start | 95,848,647 |
| Deletion End | 95,837,554 |
| Deletion Size | 11,094 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,848,097 | 95,848,115 | GGGTCAGACCTGGGACTTC |
| TUP | 95,848,072 | 95,848,095 | TGCACGCTAGGCAAGCACTCTACC |
| TUR | 95,848,044 | 95,848,062 | ATGCAGGGACTAGGGTTGG |
| TDF | 95,837,885 | 95,837,905 | AGGGAGACTTGCCTTCCACTG |
| TDP | 95,837,860 | 95,837,884 | TGGTGGTCCGTCTTCAGCATTAGCC |
| TDR | 95,837,785 | 95,837,805 | CCAGGCTTCCCAAGGACTTTC |
PCR Primers
| Primer | Sequence |
| SU | TGACGTGTGTCTGTCTGCTG |
| SD | GTCAGCAGCTCCTTTGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 121 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15019A-B5 | VGB6 | C57BL/6NTac | |
| 15019A-G11 | VGB6 | C57BL/6NTac | |
| 15019A-D8 | VGB6 | C57BL/6NTac | |
| 15019A-E10 | VGB6 | C57BL/6NTac | |
| 15019A-E9 | VGB6 | C57BL/6NTac | |
| 15019A-C9 | VGB6 | C57BL/6NTac | |
