| Gene | Slc41a1 |
| Chromosome | 1 |
| Deletion Start | 131,830,625 |
| Deletion End | 131,846,829 |
| Deletion Size | 16,205 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 131,830,943 | 131,830,961 | GCAGGCTTTGGGACTGTGG |
| TUP | 131,830,969 | 131,830,994 | CATGGTGCTGGACATTGTGCAGGTAA |
| TUR | 131,831,017 | 131,831,039 | GGTATTGAGCAAGGGCTTGTGAG |
| TDF | 131,845,907 | 131,845,927 | AACATGCCGATTCCCATTCAC |
| TDP | 131,845,932 | 131,845,955 | CCCAGCGTTAGGCCTGAGGTTGTA |
| TDR | 131,845,957 | 131,845,978 | CAGTGGACAGTGGGTCACAAAC |
PCR Primers
| Primer | Sequence |
| SU | TGCATCTGGTGAGGACTTTG |
| SD | AGCCAAACCCTAAACAACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15020A-E4 | VGB6 | C57BL/6NTac | |
| 15020A-E2 | VGB6 | C57BL/6NTac | |
| 15020A-C8 | VGB6 | C57BL/6NTac | |
| 15020A-E1 | VGB6 | C57BL/6NTac | |
