| Gene | Slc41a2 |
| Chromosome | 10 |
| Deletion Start | 83,316,606 |
| Deletion End | 83,297,158 |
| Deletion Size | 19,449 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,316,175 | 83,316,198 | AGCGAGGGCGATGATGTTATTGTG |
| TUP | 83,316,144 | 83,316,170 | CAAGCCAGAGGATTCCAAAGGAGTCCA |
| TUR | 83,316,093 | 83,316,113 | CAAACCCAGCCAGCAAGAATG |
| TDF | 83,297,242 | 83,297,263 | GACTGGCATAAACCCTGACAAC |
| TDP | 83,297,220 | 83,297,240 | TGGCCACACCCATTGCTGCTA |
| TDR | 83,297,166 | 83,297,187 | ACAAACCCTGACTTATCCAAGC |
PCR Primers
| Primer | Sequence |
| SU | ATGTCACAGAACAGATCTGTG |
| SD | TGTCTGAGGCTGCATGACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15021A-F1 | VGB6 | C57BL/6NTac | |
| 15021A-H9 | VGB6 | C57BL/6NTac | |
| 15021A-H7 | VGB6 | C57BL/6NTac | |
| 15021A-D7 | VGB6 | C57BL/6NTac | |
