| Gene | Slc43a2 |
| Chromosome | 11 |
| Deletion Start | 75,532,632 |
| Deletion End | 75,572,546 |
| Deletion Size | 39,915 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,533,001 | 75,533,021 | CCATTTGCCCAAGCTGTAGAC |
| TUP | 75,533,026 | 75,533,052 | AGACTAATAGACTCCTGCGTGCAGTCA |
| TUR | 75,533,067 | 75,533,085 | GGCCCTGGAAGACCAGAAG |
| TDF | 75,572,054 | 75,572,076 | CCATCCAAAGCATTCTCATCCAG |
| TDP | 75,572,078 | 75,572,103 | TGTCATGAGTTGTCCCAATCCCAGCC |
| TDR | 75,572,140 | 75,572,161 | TCAACCCACATTCCAGCATCTC |
PCR Primers
| Primer | Sequence |
| SU | AGCTGCCATGCCTCAGTTTA |
| SD | AGGGACAGGTAGTGTGCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15024A-A5 | VGB6 | C57BL/6NTac | |
| 15024A-F11 | VGB6 | C57BL/6NTac | |
| 15024A-B10 | VGB6 | C57BL/6NTac | |
| 15024A-E4 | VGB6 | C57BL/6NTac | |
| 15024A-C11 | VGB6 | C57BL/6NTac | |
| 15024A-B1 | VGB6 | C57BL/6NTac | |
