| Gene | Slc43a3 |
| Chromosome | 2 |
| Deletion Start | 84,937,689 |
| Deletion End | 84,957,842 |
| Deletion Size | 20,154 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,938,296 | 84,938,317 | TCCCACTGGCTACATCTTTGAC |
| TUP | 84,938,321 | 84,938,343 | TTCAAGACTACTGTGGCCCGCCT |
| TUR | 84,938,344 | 84,938,364 | GCCCAGGCTTACATGGCTATC |
| TDF | 84,957,261 | 84,957,279 | CACACGGGACCTGGGAAAG |
| TDP | 84,957,283 | 84,957,309 | ACTAGTGCCCTCTAGGCCATTGCTTCC |
| TDR | 84,957,324 | 84,957,346 | CACATGCCAGCCTCTATTAAACC |
PCR Primers
| Primer | Sequence |
| SU | CGGGTTCTGTGAATGAAAGG |
| SD | AGGAGACATTGCTCACAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15025A-F8 | VGB6 | C57BL/6NTac | |
| 15025A-H2 | VGB6 | C57BL/6NTac | |
