| Gene | Slc44a2 |
| Chromosome | 9 |
| Deletion Start | 21,337,929 |
| Deletion End | 21,353,870 |
| Deletion Size | 15,942 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,338,145 | 21,338,163 | CGAGGACCTGACCCACAAG |
| TUP | 21,338,166 | 21,338,192 | TCCCTAGAGCACTTGAACCTCTCCACA |
| TUR | 21,338,202 | 21,338,223 | TACAAAGCAGAGCAAGGAACAC |
| TDF | 21,353,166 | 21,353,185 | AGACCGGGTTTGTGCATGTG |
| TDP | 21,353,186 | 21,353,208 | ACAGCGGCGCTTCTTTGACTGGG |
| TDR | 21,353,261 | 21,353,280 | GGACTCAGGGAGAGGATGAC |
PCR Primers
| Primer | Sequence |
| SU | GCAGGGTGAAATAGTCTGGG |
| SD | TGAGACGTGTATTTGCTGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 115 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15027A-A2 | VGB6 | C57BL/6NTac | |
| 15027A-G9 | VGB6 | C57BL/6NTac | |
| 15027A-G8 | VGB6 | C57BL/6NTac | |
| 15027A-E5 | VGB6 | C57BL/6NTac | |
| 15027A-F12 | VGB6 | C57BL/6NTac | |
| 15027A-G1 | VGB6 | C57BL/6NTac | |
