| Gene | Slc44a4 |
| Chromosome | 17 |
| Deletion Start | 34,914,490 |
| Deletion End | 34,930,287 |
| Deletion Size | 15,798 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,914,595 | 34,914,616 | TCCTGACAGAGGAACTGGAGTC |
| TUP | 34,914,622 | 34,914,645 | TCTCTGAGGAGCGGCTACAGGAAA |
| TUR | 34,914,669 | 34,914,690 | CCATCCTTCCAGGACCTGATTC |
| TDF | 34,929,717 | 34,929,739 | TTTGCCTCCCAAGCAATAGAATC |
| TDP | 34,929,741 | 34,929,769 | TGGATATGCGCAACCATTCTCAAGACAGA |
| TDR | 34,929,770 | 34,929,788 | GAGGTCTCCACAGCACAAC |
PCR Primers
| Primer | Sequence |
| SU | CAGTTGGGTAGGAAGCAAGC |
| SD | GCCTCAGTCACCGAACAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15029A-C9 | VGB6 | C57BL/6NTac | |
| 15029A-D3 | VGB6 | C57BL/6NTac | |
| 15029A-E5 | VGB6 | C57BL/6NTac | |
| 15029A-F6 | VGB6 | C57BL/6NTac | |
| 15029A-H4 | VGB6 | C57BL/6NTac | |
| 15029A-E9 | VGB6 | C57BL/6NTac | |
