| Gene | Slc45a1 |
| Chromosome | 4 |
| Deletion Start | 150,644,341 |
| Deletion End | 150,629,508 |
| Deletion Size | 14,834 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 150,644,248 | 150,644,269 | TCTCCCATCTCCAGCTACTCAG |
| TUP | 150,644,223 | 150,644,245 | TCCGTGACAGGACACATCAGCCA |
| TUR | 150,644,196 | 150,644,216 | GGGTGGCGTTTGAAGTTGTTG |
| TDF | 150,629,814 | 150,629,834 | GTGGCAGGTTTGACTGATGTG |
| TDP | 150,629,789 | 150,629,812 | CTGTCTCCCGGTCAGTTTGCAGGG |
| TDR | 150,629,733 | 150,629,752 | CAGCTAAGCAGCGAGATGTC |
PCR Primers
| Primer | Sequence |
| SU | CTGATCTCCTGCTCCCTGAC |
| SD | GGGCTAGCTGAACCTTTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15030A-B11 | VGB6 | C57BL/6NTac | |
| 15030B-C8 | VGB6 | C57BL/6NTac | |
| 15030C-E11 | VGB6 | C57BL/6NTac | |
