| Gene | Slc46a3 |
| Chromosome | 5 |
| Deletion Start | 147,894,018 |
| Deletion End | 147,878,918 |
| Deletion Size | 15,101 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 147,893,237 | 147,893,258 | CAGGCTCTGTCAAGCAGGATTC |
| TUP | 147,893,205 | 147,893,233 | AGACGTCAGCTGTTTATCTACAGGGCTGC |
| TUR | 147,893,157 | 147,893,179 | TGTTGCCACCAAATATGCCTAAG |
| TDF | 147,879,760 | 147,879,782 | TGGGATTTACTCAGCCACTGTTG |
| TDP | 147,879,733 | 147,879,758 | CTGGTACCCTGGCTTCATCTTCCTGC |
| TDR | 147,879,679 | 147,879,701 | CGAGAGCTACCTACCATAGACTG |
PCR Primers
| Primer | Sequence |
| SU | ACTTAGTCCCTGCTCCTCGG |
| SD | AATCACATGGGAGACTGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15032A-G4 | VGB6 | C57BL/6NTac | |
| 15032A-A10 | VGB6 | C57BL/6NTac | |
| 15032A-F10 | VGB6 | C57BL/6NTac | |
| 15032A-F9 | VGB6 | C57BL/6NTac | |
| 15032A-F5 | VGB6 | C57BL/6NTac | |
| 15032A-H5 | VGB6 | C57BL/6NTac | |
