| Gene | Slc47a1 |
| Chromosome | 11 |
| Deletion Start | 61,366,642 |
| Deletion End | 61,344,250 |
| Deletion Size | 22,393 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,366,209 | 61,366,229 | GAGAGGACCTTGGCAAGGAAG |
| TUP | 61,366,179 | 61,366,204 | CAGCTCTAGACCTCCTTTGGCCTTGA |
| TUR | 61,366,146 | 61,366,166 | CAACTGGAGACGTGCAGGAAG |
| TDF | 61,344,439 | 61,344,458 | GCCGATGAACCAGCAACAAG |
| TDP | 61,344,411 | 61,344,435 | TGCCTATCCGTCCAAAGGACAGCAA |
| TDR | 61,344,367 | 61,344,386 | GCAGGAGTCCTCTCCGTAAG |
PCR Primers
| Primer | Sequence |
| SU | GGTGGTGGGAACAACATTTC |
| SD | CGAATACTGGTATTTGGTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15033A-B4 | VGB6 | C57BL/6NTac | |
| 15033A-D1 | VGB6 | C57BL/6NTac | |
| 15033A-E5 | VGB6 | C57BL/6NTac | |
| 15033A-F9 | VGB6 | C57BL/6NTac | |
| 15033A-F11 | VGB6 | C57BL/6NTac | |
| 15033A-H2 | VGB6 | C57BL/6NTac | |
