| Gene | Slc47a2 |
| Chromosome | 11 |
| Deletion Start | 61,342,775 |
| Deletion End | 61,302,148 |
| Deletion Size | 40,628 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,342,481 | 61,342,502 | CAGTTCCATCTTCTGCGGACAC |
| TUP | 61,342,451 | 61,342,475 | CAAAGTCGAGTTGGACGCCGTGACA |
| TUR | 61,342,397 | 61,342,418 | TCCGGCTTTACCACATCATCAG |
| TDF | 61,302,259 | 61,302,278 | GGGATGGCTCTGACTGTTTC |
| TDP | 61,302,230 | 61,302,255 | TGCTGTCCTCATAGCAGGGATTGTGG |
| TDR | 61,302,197 | 61,302,219 | TCCTATCTTAGCCACGGTCATTG |
PCR Primers
| Primer | Sequence |
| SU | GCTTGAACAAAGTCGAAGCC |
| SD | TTTCCTACCAGCAGAGCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15034A-A12 | VGB6 | C57BL/6NTac | |
| 15034A-H6 | VGB6 | C57BL/6NTac | |
| 15034A-D5 | VGB6 | C57BL/6NTac | |
| 15034A-B5 | VGB6 | C57BL/6NTac | |
| 15034A-D7 | VGB6 | C57BL/6NTac | |
