| Gene | Slc5a10 |
| Chromosome | 11 |
| Deletion Start | 61,719,949 |
| Deletion End | 61,709,283 |
| Deletion Size | 10,667 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,719,282 | 61,719,304 | GAGACCCGCTATCCCTTTCTTTC |
| TUP | 61,719,251 | 61,719,280 | TCCTATAAGTCCTGTTCCAAGACAAGAGCC |
| TUR | 61,719,199 | 61,719,219 | GCCGTCCCTACCCTACAATAC |
| TDF | 61,709,667 | 61,709,685 | CGCAGGAGCCCTGTTTGTC |
| TDP | 61,709,643 | 61,709,666 | CACATCTGCCTGGGCTGGAACTTC |
| TDR | 61,709,613 | 61,709,631 | AATGGCGAGCGTGAGGATG |
PCR Primers
| Primer | Sequence |
| SU | ACTCCAGAAGCAAGCCTGTC |
| SD | TGCAGGCTTTTGTCTGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15035A-D12 | VGB6 | C57BL/6NTac | |
| 15035A-F4 | VGB6 | C57BL/6NTac | |
| 15035A-A6 | VGB6 | C57BL/6NTac | |
