| Gene | Slc5a4a |
| Chromosome | 10 |
| Deletion Start | 76,147,534 |
| Deletion End | 76,189,333 |
| Deletion Size | 41,800 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,148,369 | 76,148,388 | GAAGACGAACCGAAGCACTG |
| TUP | 76,148,390 | 76,148,415 | TGGAGGCTTCTTTCTGGCAGGTCGAT |
| TUR | 76,148,433 | 76,148,454 | CAGAAACCTCAGACCCACTTAC |
| TDF | 76,189,150 | 76,189,170 | GGAGGAAGCTCTGACAGACAC |
| TDP | 76,189,173 | 76,189,195 | CAGAGAAGCCCTTCTGGCGGACG |
| TDR | 76,189,211 | 76,189,230 | ACAGCCAAGAGGAGGATGAC |
PCR Primers
| Primer | Sequence |
| SU | GTCCTGTCCTGGCTTCTCAC |
| SD | AGGATTTCAGACCCTTTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15036A-A2 | VGB6 | C57BL/6NTac | |
| 15036A-A3 | VGB6 | C57BL/6NTac | |
| 15036A-A6 | VGB6 | C57BL/6NTac | |
| 15036A-E1 | VGB6 | C57BL/6NTac | |
| 15036A-B6 | VGB6 | C57BL/6NTac | |
