| Gene | Slc7a5 |
| Chromosome | 8 |
| Deletion Start | 121,907,603 |
| Deletion End | 121,883,011 |
| Deletion Size | 24,593 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 121,907,442 | 121,907,462 | CGCAATATCACGCTGCTCAAC |
| TUP | 121,907,416 | 121,907,437 | TGGCCATCATCGTGGGCACCAT |
| TUR | 121,907,390 | 121,907,409 | GCGTCACGAAGATGCCAGAG |
| TDF | 121,883,631 | 121,883,652 | GCCTGTCTACTTCTTTGGTGTC |
| TDP | 121,883,605 | 121,883,630 | TGGTGGAAGAACAAGCCCAAGTGGAT |
| TDR | 121,883,561 | 121,883,581 | ACTGGTATGGGCAGTGGTGTG |
PCR Primers
| Primer | Sequence |
| SU | TGCACAGGACTTGTGAGGAG |
| SD | AAACTTGACCCAAATGCACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15039B-H3 | VGB6 | C57BL/6NTac | |
| 15039C-C3 | VGB6 | C57BL/6NTac | |
| 15039C-D8 | VGB6 | C57BL/6NTac | |
