| Gene | Slc9a8 |
| Chromosome | 2 |
| Deletion Start | 167,421,755 |
| Deletion End | 167,451,345 |
| Deletion Size | 29,591 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 167,422,254 | 167,422,273 | TGGGTAGCATGAGGGAAGAG |
| TUP | 167,422,278 | 167,422,302 | CCACCCTAGGTGGGAGAAAGACGTC |
| TUR | 167,422,305 | 167,422,324 | GATTTCAAGCTGCGGGTCAC |
| TDF | 167,450,554 | 167,450,578 | GTGCTCCTATGCCTAGTTTATGTTG |
| TDP | 167,450,581 | 167,450,603 | CGGGATCAGACTGAGGGCCTCAA |
| TDR | 167,450,637 | 167,450,658 | AGCCTCAGGCCAGATTAGAAAG |
PCR Primers
| Primer | Sequence |
| SU | TTCCTCCAAATCTTTCCCG |
| SD | TAGCTGCCTGGCTGTCACA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15042A-B2 | VGB6 | C57BL/6NTac | |
| 15042A-B11 | VGB6 | C57BL/6NTac | |
| 15042A-G2 | VGB6 | C57BL/6NTac | |
| 15042A-E5 | VGB6 | C57BL/6NTac | |
| 15042A-G5 | VGB6 | C57BL/6NTac | |
