| Gene | Tmigd1 |
| Chromosome | 11 |
| Deletion Start | 76,904,571 |
| Deletion End | 76,916,076 |
| Deletion Size | 11,506 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,905,331 | 76,905,354 | GGCCAGCAGAGCTTAGATAGAAAG |
| TUP | 76,905,357 | 76,905,382 | TCTGGTTCAGTGTGAGACTCTGCTCA |
| TUR | 76,905,413 | 76,905,431 | CAGAGGAGGGCATCAGGTG |
| TDF | 76,915,827 | 76,915,846 | GCCAAGGCTGTACCATGTTC |
| TDP | 76,915,847 | 76,915,871 | CCACAGCAAGCCCTGCATCCTGTAT |
| TDR | 76,915,895 | 76,915,912 | GCCATGCGCTGGCTAAAG |
PCR Primers
| Primer | Sequence |
| SU | CTTTGCTCAGTTAGGGCCAC |
| SD | TTGCATCTTTATTGGCTCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15048A-H3 | VGB6 | C57BL/6NTac | |
| 15048A-D4 | VGB6 | C57BL/6NTac | |
| 15048A-E4 | VGB6 | C57BL/6NTac | |
