15056   Tsnax     Status: ES cell colonies screened / QC positives

GeneTsnax
Chromosome8
Deletion Start125,013,200
Deletion End125,032,951
Deletion Size19,752
Genome Build38
MGI IDMGI:1855672
NCBI Gene ID53424
BAC ID116M14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 8

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF125,013,474125,013,491GCAAGGGCTCCATCCATC
TUP125,013,501125,013,524CTGCCTAAGGACCAGGAGGGTTCA
TUR125,013,525125,013,544GGTGTCATGCTTCCTCTTCC
TDF125,032,675125,032,696ACGATGGGTTCTCGTTCATTGG
TDP125,032,697125,032,725CAACACTGGGCCCTATGAGGTCTCTAAGA
TDR125,032,736125,032,754GGCCAGGCTCTGTTTCAAG

PCR Primers

PrimerSequence
SUTAGGCACTTGGACGTGGGA
SDATCCTGCTGAGGAACGTAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR71 bp++-
TDFTDR80 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
15056A-C3VGB6C57BL/6NTac
15056A-E3VGB6C57BL/6NTac
15056A-F2VGB6C57BL/6NTac
15056A-F7VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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