| Gene | Trpm5 |
| Chromosome | 7 |
| Deletion Start | 143,089,605 |
| Deletion End | 143,072,263 |
| Deletion Size | 17,343 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 143,088,975 | 143,088,995 | GTGGGTGAGGAACGACCTTTG |
| TUP | 143,088,947 | 143,088,973 | CTATGAAGTCGTGGCTTCGGGATGTCC |
| TUR | 143,088,910 | 143,088,930 | CCTGTGCTCTGAGCTGCTTTC |
| TDF | 143,072,947 | 143,072,967 | GCACCACATCCGGTCACTATC |
| TDP | 143,072,919 | 143,072,946 | CCTTATCATGCACTATGGGAGGACCTGG |
| TDR | 143,072,897 | 143,072,916 | CCCTGGGACTCCACACTCTG |
PCR Primers
| Primer | Sequence |
| SU | TGTCTTTGGAGGTTTCCAGG |
| SD | AGCTAATGAGTGGGTCAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15060B-D6 | VGB6 | C57BL/6NTac | |
| 15060B-C3 | VGB6 | C57BL/6NTac | |
| 15060B-E10 | VGB6 | C57BL/6NTac | |
