| Gene | Wnt11 |
| Chromosome | 7 |
| Deletion Start | 98,839,111 |
| Deletion End | 98,853,340 |
| Deletion Size | 14,230 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 98,839,980 | 98,840,003 | GTTTGGCAGGTAGGAGATATGGAG |
| TUP | 98,840,005 | 98,840,029 | ACACTCTCAGAGTACAGTGCCCGGA |
| TUR | 98,840,049 | 98,840,070 | CCTGAGCAAGGCAGACACATTC |
| TDF | 98,853,113 | 98,853,134 | GCAGTGCAACAAGACTTCCAAC |
| TDP | 98,853,138 | 98,853,163 | AGTGACAGCTGCGACCTCATGTGCTG |
| TDR | 98,853,189 | 98,853,208 | CATCGCTCCACCACTCTGTC |
PCR Primers
| Primer | Sequence |
| SU | TAGGGTCTCACCTAATCCGA |
| SD | TTAGTGGTTCCATGTGGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15062A-B4 | VGB6 | C57BL/6NTac | |
| 15062A-B11 | VGB6 | C57BL/6NTac | |
| 15062A-C1 | VGB6 | C57BL/6NTac | |
| 15062A-D2 | VGB6 | C57BL/6NTac | |
| 15062A-E7 | VGB6 | C57BL/6NTac | |
| 15062A-A11 | VGB6 | C57BL/6NTac | |
