| Gene | O3far1 |
| Chromosome | 19 |
| Deletion Start | 38,097,130 |
| Deletion End | 38,114,096 |
| Deletion Size | 16,967 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,097,191 | 38,097,210 | GCACCCACTTCCCTTTCTTC |
| TUP | 38,097,211 | 38,097,232 | TCGGATGTCAAGGGCGACCACC |
| TUR | 38,097,238 | 38,097,256 | TCTCCACGACGCTCAACAC |
| TDF | 38,113,619 | 38,113,637 | CGAAAGCATCGCGGAAGAG |
| TDP | 38,113,641 | 38,113,666 | TACGCTGAGCTTGGCATACTCTGAGA |
| TDR | 38,113,693 | 38,113,710 | GCGGAAGAGTCGGTAGTC |
PCR Primers
| Primer | Sequence |
| SU | TTAAGAGCACTTAGACTGCC |
| SD | AGCCACACTACGATTGAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15078A-A9 | VGB6 | C57BL/6NTac | |
| 15078B-F5 | VGB6 | C57BL/6NTac | |
| 15078C-H7 | VGB6 | C57BL/6NTac | |
