| Gene | Trpv2 |
| Chromosome | 11 |
| Deletion Start | 62,578,411 |
| Deletion End | 62,600,224 |
| Deletion Size | 21,814 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,579,234 | 62,579,257 | GGCTGGACTGATGGTGATAGAATG |
| TUP | 62,579,262 | 62,579,289 | CCACAATGCCCTTTAGAATCCCAGTCGG |
| TUR | 62,579,292 | 62,579,313 | AGACTAGGGAAGCACAGAAAGG |
| TDF | 62,599,394 | 62,599,415 | CAGCAGGTAGTGTGCATGTAAC |
| TDP | 62,599,421 | 62,599,444 | CCTTCACACCTAGACGTGGGCCCT |
| TDR | 62,599,462 | 62,599,480 | GGCCAAGGGTCAGCATCAG |
PCR Primers
| Primer | Sequence |
| SU | TGACCTTGAACTTCTGATCCTC |
| SD | TGGCTCTTAGAGACACATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15082A-A5 | VGB6 | C57BL/6NTac | |
| 15082A-D10 | VGB6 | C57BL/6NTac | |
| 15082A-G7 | VGB6 | C57BL/6NTac | |
| 15082A-A7 | VGB6 | C57BL/6NTac | |
| 15082A-C11 | VGB6 | C57BL/6NTac | |
