| Gene | Vash1 |
| Chromosome | 12 |
| Deletion Start | 86,679,991 |
| Deletion End | 86,691,928 |
| Deletion Size | 11,938 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,680,815 | 86,680,832 | TTCCCACGCCAGGGTTTG |
| TUP | 86,680,837 | 86,680,859 | TCCTTCTGCTCAGCTCTGGTGGG |
| TUR | 86,680,864 | 86,680,885 | CACCGCTCATGTGACTGAATCC |
| TDF | 86,691,253 | 86,691,272 | CTGCAAGGGCTCTGCGTATG |
| TDP | 86,691,273 | 86,691,295 | ACCAGAGGCTGAGCAGCCAGGAC |
| TDR | 86,691,339 | 86,691,359 | TTGCCATTTGGACACAGACAC |
PCR Primers
| Primer | Sequence |
| SU | ATCGTAGATCGCTGTGGGTC |
| SD | AAGGAGCACCACAGAACGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15083A-F8 | VGB6 | C57BL/6NTac | |
| 15083B-H8 | VGB6 | C57BL/6NTac | |
| 15083B-E8 | VGB6 | C57BL/6NTac | |
| 15083B-F4 | VGB6 | C57BL/6NTac | |
