| Gene | Plxnb1 |
| Chromosome | 9 |
| Deletion Start | 109,100,081 |
| Deletion End | 109,117,957 |
| Deletion Size | 17,877 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,100,947 | 109,100,970 | GGACGTACTCTTTGCAGCTTTCTC |
| TUP | 109,100,976 | 109,100,998 | TGGCTCCTCCCACTGTGGATTGG |
| TUR | 109,101,027 | 109,101,046 | GGCACAGAGCACAGAGGTTC |
| TDF | 109,117,542 | 109,117,559 | TGGTGAGTGGCGAGTGTC |
| TDP | 109,117,561 | 109,117,585 | TTCCCTGGCAGCCTGTTCTTGAACG |
| TDR | 109,117,611 | 109,117,629 | TCCTGCCTCTCCAGTGTTG |
PCR Primers
| Primer | Sequence |
| SU | CAAAGAGCAGGGTTTGGAAG |
| SD | CCCAATGGGACATAGGTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15084A-B2 | VGB6 | C57BL/6NTac | |
| 15084A-E12 | VGB6 | C57BL/6NTac | |
| 15084A-F1 | VGB6 | C57BL/6NTac | |
| 15084A-H2 | VGB6 | C57BL/6NTac | |
| 15084A-G9 | VGB6 | C57BL/6NTac | |
| 15084A-F6 | VGB6 | C57BL/6NTac | |
