| Gene | Plat |
| Chromosome | 8 |
| Deletion Start | 22,767,108 |
| Deletion End | 22,782,116 |
| Deletion Size | 15,009 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,767,138 | 22,767,158 | TGTGGACTGGCTTTCCCATTG |
| TUP | 22,767,161 | 22,767,183 | TGACCAGGTGGGTCTACCTCCCT |
| TUR | 22,767,214 | 22,767,234 | CCTGGTTACACCACCGTTATC |
| TDF | 22,781,914 | 22,781,936 | CGCGTACAAACCACTGACCTATG |
| TDP | 22,781,938 | 22,781,959 | TCTTCCACAGGGTGACTCGGGA |
| TDR | 22,782,025 | 22,782,044 | GCACATCCTTCTGCCCACAG |
PCR Primers
| Primer | Sequence |
| SU | CACAAAATCTCTCCTCCCAC |
| SD | CTGTGATTCGGCCAGATACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 131 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15085A-A11 | VGB6 | C57BL/6NTac | |
| 15085A-B4 | VGB6 | C57BL/6NTac | |
| 15085A-D1 | VGB6 | C57BL/6NTac | |
| 15085A-F11 | VGB6 | C57BL/6NTac | |
| 15085A-E8 | VGB6 | C57BL/6NTac | |
| 15085A-H11 | VGB6 | C57BL/6NTac | |
