| Gene | Trpm2 |
| Chromosome | 10 |
| Deletion Start | 77,967,009 |
| Deletion End | 77,929,219 |
| Deletion Size | 37,791 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,966,791 | 77,966,812 | CAGAGGCAGCTCAATGGTCATC |
| TUP | 77,966,763 | 77,966,788 | TGGTTAGGTGGTCCATTCATGCTGCC |
| TUR | 77,966,694 | 77,966,712 | CCGCCTGTCTGAGTTGTTC |
| TDF | 77,929,981 | 77,929,998 | TGTGCAGAGGCTGAGGTG |
| TDP | 77,929,959 | 77,929,979 | TACCCTGCCTACCGCCAGGCT |
| TDR | 77,929,891 | 77,929,913 | GACGGAGACAGAACATGATGAAG |
PCR Primers
| Primer | Sequence |
| SU | GAGCTTGTAGACTGTCCAAG |
| SD | CTTAAGTTCCTGGTCACACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 119 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15087A-A7 | VGB6 | C57BL/6NTac | |
| 15087A-A12 | VGB6 | C57BL/6NTac | |
| 15087A-G8 | VGB6 | C57BL/6NTac | |
| 15087A-H8 | VGB6 | C57BL/6NTac | |
