| Gene | Nrtn |
| Chromosome | 17 |
| Deletion Start | 56,752,474 |
| Deletion End | 56,751,369 |
| Deletion Size | 1,106 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,752,068 | 56,752,088 | GTCTCTTGTAGCCCAGAGCAG |
| TUP | 56,752,037 | 56,752,064 | CAAACTCACAGAGTACCAGAGGATGCCC |
| TUR | 56,752,015 | 56,752,036 | GGAGGCAGGAGGATTACTTCAG |
| TDF | 56,751,484 | 56,751,502 | GGCCTATGAGGACGAGGTG |
| TDP | 56,751,461 | 56,751,482 | CCTTCCTGGACGTGCACAGCCG |
| TDR | 56,751,441 | 56,751,460 | GCTCTTGCAGCGTGTGGTAG |
PCR Primers
| Primer | Sequence |
| SU | AATGCAAATGTCTTCCAGGC |
| SD | TATCCTGGGCCTGTGCTATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 62 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15091A-H6 | VGB6 | C57BL/6NTac | |
| 15091A-B5 | VGB6 | C57BL/6NTac | |
| 15091A-F10 | VGB6 | C57BL/6NTac | |
| 15091A-C4 | VGB6 | C57BL/6NTac | |
| 15091A-G3 | VGB6 | C57BL/6NTac | |
