| Gene | Uncx |
| Chromosome | 5 |
| Deletion Start | 139,543,997 |
| Deletion End | 139,547,792 |
| Deletion Size | 3,796 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,544,536 | 139,544,556 | AGCTGGAGAAGGCGTTCAATG |
| TUP | 139,544,559 | 139,544,584 | AGCCACTACCCGGACGTGTTTATGCG |
| TUR | 139,544,636 | 139,544,653 | CCTTGGGCGACACCAATC |
| TDF | 139,547,300 | 139,547,321 | CAAGGGCCACTTCTTGCTCTAC |
| TDP | 139,547,336 | 139,547,359 | CCTGGGTTTCCTAGTGCCACAGGC |
| TDR | 139,547,383 | 139,547,403 | CGTCCTTGGGCACCAATTCAG |
PCR Primers
| Primer | Sequence |
| SU | TTATGGAGGTGCGTGTCAGG |
| SD | TTGGTCCCCATCGACACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15092A-F10 | VGB6 | C57BL/6NTac | |
| 15092A-B11 | VGB6 | C57BL/6NTac | |
