15093   Cd27     Status: ES cell colonies screened / QC positives

GeneCd27
Chromosome6
Deletion Start125,236,889
Deletion End125,233,237
Deletion Size3,653
Genome Build38
MGI IDMGI:88326
NCBI Gene ID21940
BAC ID263K13
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF125,236,193125,236,214CAGTCACGCACGTCCATAATCC
TUP125,236,168125,236,192CAACTACAAGGCAGGCTGAGGCAGG
TUR125,236,135125,236,157CCTGGCCTTGAACTCCTATCATC
TDF125,233,975125,233,993TCCAGGTCAGGGAGGAATC
TDP125,233,944125,233,973TGTCTCAAGGGACTAAACTAGACTCCGAGA
TDR125,233,922125,233,941GCTCTGTCTGTCTCTGTGTC

PCR Primers

PrimerSequence
SUGAGAGAGGACACTTGCCAGG
SDTGGTGAGCCATTTCCACCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR80 bp++-
TDFTDR72 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
15093A-G3VGB6C57BL/6NTac
15093A-B2VGB6C57BL/6NTac
15093A-C12VGB6C57BL/6NTac
15093A-D4VGB6C57BL/6NTac
15093A-F11VGB6C57BL/6NTac
15093A-D11VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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