| Gene | Pspn |
| Chromosome | 17 |
| Deletion Start | 57,000,012 |
| Deletion End | 56,999,454 |
| Deletion Size | 559 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,999,968 | 56,999,987 | GTCTGCTGCTCCTGTCCTTG |
| TUP | 56,999,941 | 56,999,963 | CGAGCCTCGGCTGGGTCCTTGAT |
| TUR | 56,999,880 | 56,999,902 | TCCCTCTAGTCTCTGCCATCTTC |
| TDF | 56,999,661 | 56,999,680 | CCTCGGAGGAGAAGGTCATC |
| TDP | 56,999,636 | 56,999,659 | TCCGATACTGTGCTGGCAGCTGTC |
| TDR | 56,999,603 | 56,999,624 | GTACCAGACTGTGCTGGGTATG |
PCR Primers
| Primer | Sequence |
| SU | GGTAGCCAGCTCTGGACTTG |
| SD | TGACCTCCCACATATCCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15094A-C4 | VGB6 | C57BL/6NTac | |
| 15094B-D2 | VGB6 | C57BL/6NTac | |
| 15094B-G11 | VGB6 | C57BL/6NTac | |
| 15094B-H9 | VGB6 | C57BL/6NTac | |
| 15094C-E2 | VGB6 | C57BL/6NTac | |
| 15094C-H9 | VGB6 | C57BL/6NTac | |
| 15094B-A12 | VGB6 | C57BL/6NTac | |
