| Gene | Sema4f |
| Chromosome | 6 |
| Deletion Start | 82,939,620 |
| Deletion End | 82,913,464 |
| Deletion Size | 26,157 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 82,939,434 | 82,939,455 | ATGCAGGAACCCTTGTCCTCAG |
| TUP | 82,939,410 | 82,939,431 | CTTCGCACGGTACCCTGCCCTT |
| TUR | 82,939,352 | 82,939,372 | GGCCTTAGATGGCTGTCACTG |
| TDF | 82,913,649 | 82,913,669 | ATAGCCAAGACCCTCCCTCTC |
| TDP | 82,913,623 | 82,913,646 | TCACCTGAAGATGAACGGCTGCCC |
| TDR | 82,913,547 | 82,913,565 | CTGGGCTCGGGCAAGAATC |
PCR Primers
| Primer | Sequence |
| SU | GGAACGTTTCCATCCTTCTG |
| SD | CAAAGGGAAACTGAGTTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 123 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15095A-E6 | VGB6 | C57BL/6NTac | |
| 15095B-F6 | VGB6 | C57BL/6NTac | |
| 15095C-B10 | VGB6 | C57BL/6NTac | |
| 15095C-D4 | VGB6 | C57BL/6NTac | |
| 15095C-D11 | VGB6 | C57BL/6NTac | |
| 15095C-E12 | VGB6 | C57BL/6NTac | |
