| Gene | Gfra4 |
| Chromosome | 2 |
| Deletion Start | 131,042,679 |
| Deletion End | 131,040,150 |
| Deletion Size | 2,530 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 131,041,931 | 131,041,955 | GAATACTGCATCCATTCACATCCTG |
| TUP | 131,041,897 | 131,041,924 | TAGATATCCCTAGTGCCTGCTCATGCCC |
| TUR | 131,041,872 | 131,041,890 | GCGTGACCCGTTGATCTTG |
| TDF | 131,040,286 | 131,040,307 | GAGAAGAGCTGGAGGCAGAAAC |
| TDP | 131,040,253 | 131,040,276 | TTTGTCCTAACGCCCAAGGTGTCC |
| TDR | 131,040,223 | 131,040,242 | GAGAGCCAGGGCAGTGAGTG |
PCR Primers
| Primer | Sequence |
| SU | AGTCTGCACTGCTGCTGTTG |
| SD | TAGGTGACAAGCCACGTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15100A-A5 | VGB6 | C57BL/6NTac | |
| 15100A-C9 | VGB6 | C57BL/6NTac | |
| 15100A-C12 | VGB6 | C57BL/6NTac | |
| 15100A-G12 | VGB6 | C57BL/6NTac | |
| 15100A-E10 | VGB6 | C57BL/6NTac | |
