| Gene | Wnt7b |
| Chromosome | 15 |
| Deletion Start | 85,559,104 |
| Deletion End | 85,537,333 |
| Deletion Size | 21,772 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,558,745 | 85,558,766 | TGCAGACAGAAGCAGAGAAAGG |
| TUP | 85,558,718 | 85,558,742 | TTGATGCAGTGGGTTGGCCTATTCA |
| TUR | 85,558,667 | 85,558,687 | CCAAATGGCACCCACAGAAAG |
| TDF | 85,538,132 | 85,538,150 | CAGTCGCAGGGAGATGGAG |
| TDP | 85,538,103 | 85,538,130 | CATGGAGGGCCTGCGCTTATTAATCATC |
| TDR | 85,538,055 | 85,538,072 | AGGGCAGCAGGTAGGAAC |
PCR Primers
| Primer | Sequence |
| SU | TCTGCCATGCCAAGACAACT |
| SD | GAGTGTGACTGGTGGGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15101A-B4 | VGB6 | C57BL/6NTac | |
| 15101A-C3 | VGB6 | C57BL/6NTac | |
| 15101A-C9 | VGB6 | C57BL/6NTac | |
| 15101A-D12 | VGB6 | C57BL/6NTac | |
| 15101A-G12 | VGB6 | C57BL/6NTac | |
| 15101A-E2 | VGB6 | C57BL/6NTac | |
