| Gene | Wnt9b |
| Chromosome | 11 |
| Deletion Start | 103,749,766 |
| Deletion End | 103,730,678 |
| Deletion Size | 19,089 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,749,265 | 103,749,288 | GCGATTTCAGTATCCTTTGCTTTC |
| TUP | 103,749,245 | 103,749,264 | TCCCTGCAGAGCTGGGACCC |
| TUR | 103,749,164 | 103,749,185 | TCTCCCACAACCTTGGATCAAC |
| TDF | 103,731,095 | 103,731,117 | AGGTGCTGAAGCTACGCTATGAC |
| TDP | 103,731,071 | 103,731,093 | CGGCTGTCAAGGTGTCCAGTGCC |
| TDR | 103,731,042 | 103,731,059 | AGCTCCAGACGACCCAAG |
PCR Primers
| Primer | Sequence |
| SU | TCCGCCTTCTTTGCTGGAGT |
| SD | AGTGGGACCACAAGAGATCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 125 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15105A-G1 | VGB6 | C57BL/6NTac | |
| 15105A-D10 | VGB6 | C57BL/6NTac | |
| 15105A-C4 | VGB6 | C57BL/6NTac | |
