| Gene | Wnt8b |
| Chromosome | 19 |
| Deletion Start | 44,493,553 |
| Deletion End | 44,512,084 |
| Deletion Size | 18,532 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,493,619 | 44,493,639 | CCCGTGTGCGTTCTTCTAGTC |
| TUP | 44,493,641 | 44,493,664 | CTTGTGTCCTTCACCGCAGCCACG |
| TUR | 44,493,671 | 44,493,691 | CCACACCCAAGCAGGTTACTC |
| TDF | 44,511,266 | 44,511,284 | CAACGTGGGCTTCGGAGAG |
| TDP | 44,511,289 | 44,511,315 | TTTCCAAGCAGTTTGTGGATGCCCTCG |
| TDR | 44,511,344 | 44,511,366 | CAGCCTCATTGTTGTGCAGATTC |
PCR Primers
| Primer | Sequence |
| SU | ACTCCACGCAGTGATTCTGTG |
| SD | GAAGCTCTGGCTCACAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 15107A-F5 | VGB6 | C57BL/6NTac | |
| 15107A-B10 | VGB6 | C57BL/6NTac | |
| 15107A-H1 | VGB6 | C57BL/6NTac | |
